Fig. 3

CSP-specific IgG levels in mice after immunization with soluble or Spy-VLP displayed CSP antigen at a day 14, b day 35, c day 42, d day 148, e day 192 and f day 212 after first immunization. CSP-specific IgG levels (OD ELISA) in serum from mice (n = 6 CSP Spy-VLP group, n = 5 control vaccine group) immunized with a CSP Spy-VLP vaccine (filled circles) or with a control vaccine consisting of soluble CSP mixed with untagged AP205 VLPs (open squares). Both vaccines were formulated using aluminum hydroxide adjuvant. Mice were immunized on days 0, 14 and 42. Differences in titres between vaccination groups were analysed using OD cut-off 0.2 and Mann–Whitney Rank Sum test; day 14 (P = 0.0169), day 28 (P = 0.0109), day 42 (P = 0.0578), day 148 (P = 0.0116), days 192 and 212 (P = 0.0316)