Serological markers to measure recent changes in malaria at population level in Cambodia

Malaria Journal

Table 3 Three criteria to select the most promising antigens

	Antigens	Ags selection based on the three criteria
	Antigens	PCR⁺ vs PCR⁻ values^a	Half-lives (PCR⁺)^b	Differences in intercept^c	Sensitivity by endemicity^d
P. falciparum	CSP	+	+	+	+
	Pf13	+	+	−	+
	STARP.R	++	+	+	+
	SALSA2	+	++	+	+
	SR11.1	+	+	+	+
	LSA1.41	++	+	−	++
	LSA1.J	+	+	+	±
	LSA3.RE	++	++	++	++
	Pf.MSP1.19	+	−	−	−
	GLURP	++	++	++	++
	Pf.GLURP.R2	++	++	++	++
P. vivax	PvVK210.CSP	−	−	−	±
	PvVK247.CSP	−	−	−	±
	PvCSP	−	−	−	–
	PvAMA1	−	−	−	±
	PvEBP	++	−	++	++
	PvDBP	−	−	−	−
	Pv.MSP1.19	−	−	−	−
Vectors	SALIV1	−	−	−	−
Vectors	SALIV2	−	+	−	−

Three criteria are used to select the most promising Ags for recent malaria infection. These criteria are based on: (1) the outcome of the estimated Ab-responses based on the differences in ln(MFI) between PCR positive and PCR negative individuals, (2) the assessed half-lives with the best Ab-responses of <7.5 months followed by Ab-responses between 8.5 months until 1 year and finally (3) the sensitivity to the level of malaria endemicity in communities
^aDifference in ln(MFI) (ln-MFI PCR+ −ln-MFI PCR−); ++ (value > 1), + (value > 0.5),−(value < 0.5)
^bHalf-lives estimated via linear regression models; ++ (half-life < 7.5 months), + (half-life between 8.5 months–1 year),− (half-life > 1 year)
^cDifference in intercept between PCR+ and PCR− for half-life estimation; ++ (intercept difference > 2), + (intercept difference between 1 and 2), − (intercept difference < 1)
^dSensitivity by endemicity estimated via linear regression models; ++ (IRR of PCR prev. decline with > 0.2, IRR increase by age with >4), + (IRR of PCR prev. decline with >0.1, IRR increase by age with >1)

ISSN: 1475-2875