Fig. 1

Domain organization and conservation of K13 protein across different Plasmodium species. a The PvK12 gene is shown in red. P1F and P1R shows the primer set used for the primary PCR. P2F and P2R shows the primers used for nested PCR for the amplification of full length PvK12. All the amplification primers used are present outside the ORF. SPF1, SPR1, SPF2, SPR2, SPF3, SPR3, SPF4 and SPR4 show the primers used for sequencing. b Schematic domain organization of Kelch protein in each species showing the BTB domain and six Kelch domains. The numbers of cysteine residues in each Kelch domain is indicated. Additional Tho2 and Cdc_37 N kinase binding domains are also shown. c Percentage of sequence similarity between amino acid sequences of seven Plasmodium species. d Neighbor-Joining tree of Kelch protein amino acid sequences from seven Plasmodium species. Bootstrap values generated from 1000 replicates are shown