Fig. 2
From: Identification of a novel merozoite surface antigen of Plasmodium vivax, PvMSA180
Expression of recombinant PvMSA180. a Expression of recombinant domains of PvMSA180 in a wheat germ cell-free expression system. Arrowheads indicate the four domains of PvMSA180. D1 domain 1, D2 domain 2, D3 domain 3, and D4 domain 4. b Expression and purification of PvMSA180-D1 (GST-fused) and D4 (His-tagged) in E. coli. c Recombinant PvMSA180-D1 and D4 were probed with anti-GST and anti-His antibodies under reducing conditions. Arrowheads indicate the PvMSA180-D1 (~80 kDa) and D4 (~60 kDa) bands. d Antibody recognition of PvMSA180-D1 and D4 compare to PvDBP-RII recombinant proteins using pooled vivax-patient sera (lane P), malaria naïve healthy sera (lane H), mouse immune serum (lane M), and anti-GST or anti-His antibody (lane R)