Table 1 Pfdhfr, Pfdhps, Pfmdr1 and Pfcrt PCR primer sequences used in amplification reactions

Pfdhfr

PCR I

F

5′-TTC TCC TTT TTA TGA TGG AAC AAG T-3′

R

5′ ATA TTT GAA AAT CAT TTG GAT GTA TAG-3′

PCR II

F

5′-GTT GAA CCT AAA CGT GCT GT-3′

R

5′-TTC ATC ATG TAA TTT TTG TTG TG-3′

Multiplex

Pfdhfr51F

5′-AGG AGT ATT ACC ATG GAA ATG TA-3′

Pfdhfr16R

5′-GAC TGA CTC TCA TTT TTG CTT TCA ACC TTA CAA CAT-3′

Pfdhfr108F

5′-GAC TGA CTA CAA AAT GTT GTA GTT ATG GGA AGA ACA A-3′

Pfdhfr164R

5′-CTG ACT GAC TGA CTG ACT AAT TCT TGA TAA ACA ACG GAA CCT CCT A-3′

Pfdhfr59R

5′-CTG ACT GAC TGA CTG ACT GAC TTG ATT CAT TCA CAT ATG TTG TAA CTG CAC-3′

Pfdhps

PCR I

F

5′-TGC TTA AAT GAT ATG ATA CCC GAA TAT AAG-3′

R

5′ TCC ACC TGA AAA GAA ATA CAT AAA T-3′

PCR II

F

5′-GTT GAA CCT AAA CGT GCT GT-3′

R

5′-TTC ATC ATG TAA TTT TTG TTG TG-3′

Multiplex

Pfdhps613R

5′-TTG ATC ATT CAT GCA ATG GG-3′

Pfdhps540F

5′-GAC TGA GGA AAT CCA CAT ACA ATG GAT-3′

Pfdhps581R

5′-TAA GAG TTT AAT AGA TTG ATC ATG TTT CTT C-3′

Pfdh436-1F

5′-GAC TGA CTA GTG TTA TAG ATA TAG GTG GAG AAT CC-3′

Pfdhps436-2R

5′-GAC TGA CTG ACT GAC TTG GAT TAG GTA TAA CAA AAA GGA ICA-3′

Pfdhps437R

5′-GAC TGA CTG ACT GAC TGA CTT TTT TGG ATT AGG TAT AAC AAA AGG A-3′

Pfmdr1

PfMDR1- 1

F

5′-AGA GAA AAA AGA TGG TAA CCT CAG-3′

R

5′-ACC ACA AAC ATA AAT TAA CGG-3′

PfMDR1- 2

F

5′-CAG GAA GCA TTT TAT AAT ATG CAT-3′

R

5′-CGT TTA ACA TCT TCC AAT GTT GCA-3′

Pfcrt

PCR I

F

5′-GTT CTT GTC TTG GTA AAT GT-3′

R

5′- CGG ATG TTA CAA AAC TAT AGT T-3′

PCR II

F

5′-GTT CTT GTC TTG GTA AAT GT-3′

R

6-FAM-5′- TAA ATG TGC TCA TGT GTT TA -3′