Table 1 Primer sequences and cycling conditions for Plasmodium species identification
Genus and species | Primer name | Primer sequence (5′-3′) | PCR conditions | Reference |
|---|---|---|---|---|
Plasmodium genus | rPLU 1 rPLU 5 | TCAAAGATTAAGCCATGCAAGTGA CCTGTTGTTGCCTTAAACTTC | 95 °C for 5 min; 25× [94 °C for 1 min; 58 °C for 1 min; and 72 °C for 2 min]; 72 °C for 5 min | Snounou et al. [22] |
P. falciparum | rFAL1 rFAL2 | TTAAACTGGTTTGGGAAAACCAAATATATT ACACAATGAACTCAATCATGACTACCCGTC | 95 °C for 5 min; 30× [94 °Cfor 1 min; 58 °C for 1 min; and 72 °C for 2 min]; 72 °C for 5 min | |
P. malariae | rMAL1 rMAL2 | ATAACATAGTTGTACGTTAAGAATAACCGC AAAATTCCCATGCATAAAAAATTATACAAA | ||
P. vivax | rVIV1 rVIV2 | CGACTTCCAAGCCGAAGCAAAGAAAG TCCTTACTTCTAGCTTAATCCACATAACTGATAC | ||
P. ovale | rOVA1WC rOVA2WC | TGTAGTATTCAAACGCAGT TATGTACTTGTTAAGCCTTT | Fuehrer et al. [21] |