Table 1 Primer sequences used for nested PCR and sequencing of Plasmodium falciparum dhfr and dhps genes
Primer name | Sequences |
|---|---|
Nest 1 forward dhfr | 5′-TTTATGATGGAACAAGTCTGC-3′ |
Nest 1 reverse dhfr | 5′-AGTATATACATCGCTAACAGA-3′ |
Nest 2 forward dhfr | 5′-TCTGCGACGTTTTCGATATTT-3′ |
Nest 2 reverse dhfr | 5′-CTCATTTTCATTTATTTCTGGA-3′ |
Nest 1 forward dhps | 5′-AACCTAAACGTGCTGTTCAA-3′ |
Nest 1 reverse dhps | 5′-AATTGTGTGATTTGTCCACAA-3′ |
Nest 2 forward dhps | 5′-CCTAAACGTGCTGTTCAAAGAA-3′ |
Nest 2 reverse dhps | 5′-TTGTTCATCATGTAATTTTTGTTGTG-3′ |
Nest 2 reverse2 dhps | 5′-CAATACTTATAATTGGTTTCGCATCA-3′ (sequencing only) |
Nest 2 reverse 540 dhps | 5′-TTCGCAAATCCTAATCCAATATC-3′ (sequencing only) |