Fig. 2
From: Artemisinin resistance without pfkelch13 mutations in Plasmodium falciparum isolates from Cambodia
Pfmdr1 copy numbers are positively associated with MEF, LUM, ART and ARS. Drug sensitivity assays were set up with 0–6 h old ring stage parasites and % growth was measured 72 h later by SYBR Green. Shown are the average EC50 values from at least three biological replicates for two ART sensitive (green), two discordant (blue) and four resistant (red) isolates. Their respective clearance time, RSA0–3h percent survival and their pfmdr1 copy number are indicated at the bottom. The Spearman correlation coefficient for pfmdr1 copy number and each drug is shown in the graph. A one-way ANOVA test, with a Tukey’s post-test was performed between all isolates for each drug. Significance values are indicated by asterisks, as follows: *(p < 0.05); **(p < 0.01)