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Table 1 Candidate primer/probe combinations evaluated for gametocyte-specific targets

From: Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Gene ID

# Exons

Exons targeted

Forward primer

Probe (±strand)

Reverse primer

PF3D7_1214500 (S1)

6

2–4

TGGGATGATGAATATGAAG

TGGAACTGGTTCGCTATATTGT  (−)

AAAGGTCTATTAGTTGAATTG

PF3D7_0630000 (S3)

10

3–6

AACGCAGAAATAGAAAATC

TGGTATATTGGGAAGTGTAGAAGAG  (+)

GTCATAAGCAGATACATTC

PF3D7_0514500 (S2)

6

2–3

GATAAGACAAAACGGAAC

CACAGGAGTAGTGACCATATCAG   (+)

CTATAAGGAAAAGATAACAAAG

PF3D7_0518800 (S1)

7

1–2

GCCGTTACTGATTCCTTAC

TGTCTGAGTTACGACAAGAAATTAG (+)

CGAGAATACCCATTTGTC

PF3D7_1031000/pfs25

1

1

GAAATCCCGTTTCATACGCTTG

TGTAAGAATGTAACTTGTGGTAACGGT  (+)

AGTTTTAACAGGATTGCTTGTATCTAA

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Malaria Journal

ISSN: 1475-2875

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