Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Table 3 Summary of other PCR techniques for Plasmodium sporozoite detection

Molecular sporozoite detection approach [ref]	DNA extraction	Plasmodium species	Cycling time in min	DNA limit of detection
18s-rRNA nested PCR protocol [4, 15]	Livak or DNAzol methods	P. vivax, P. falciparum, P. ovale, P. malariae	294	0.2 ng–0.2 pg
18s-rRNA single PCR [4, 15]		P. vivax, P. falciparum, P. ovale, P. malariae	205	2 ng–4 pg
18s-rRNA Taqman assay [4]		P. falciparum, P. ovale, P. malariae, P. vivax	47	0.2 pg
18s-rRNA single PCR Tassanakajon [4, 18]		P. falciparum	60*	2 pg
Cytochrome B single PCR [22]	IsoQuick nucleic acid extraction kit	P. vivax, P. falciparum	96	0.2 pg
DHFR-TS nested [30]	Chelex	P. falciparum	>294	4–40 pg
Fast COX-I single PCR [this manuscript]	CTAB	P. vivax, P. falciparum, P. ovale s.l., P. knowlesi, P. ovale wallikeri	62	0.043 pg

min minutes, ng nanograms, pg picograms
* The original paper from Tassanakajon et al. [18] did not include times for denaturation and final extension

ISSN: 1475-2875