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Table 3 Summary of other PCR techniques for Plasmodium sporozoite detection

From: Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Molecular sporozoite detection approach [ref] DNA extraction Plasmodium species Cycling time in min DNA limit of detection
18s-rRNA nested PCR protocol [4, 15] Livak or DNAzol methods P. vivax, P. falciparum, P. ovale, P. malariae 294 0.2 ng–0.2 pg
18s-rRNA single PCR [4, 15] P. vivax, P. falciparum, P. ovale, P. malariae 205 2 ng–4 pg
18s-rRNA Taqman assay [4] P. falciparum, P. ovale, P. malariae, P. vivax 47 0.2 pg
18s-rRNA single PCR Tassanakajon [4, 18] P. falciparum 60* 2 pg
Cytochrome B single PCR [22] IsoQuick nucleic acid extraction kit P. vivax, P. falciparum 96 0.2 pg
DHFR-TS nested [30] Chelex P. falciparum >294 4–40 pg
Fast COX-I single PCR [this manuscript] CTAB P. vivax, P. falciparum, P. ovale s.l., P. knowlesi, P. ovale wallikeri 62 0.043 pg
  1. min minutes, ng nanograms, pg picograms
  2. * The original paper from Tassanakajon et al. [18] did not include times for denaturation and final extension