Fig. 3
Design of DNA prime-Ad boost protection studies. On week 0, BALB/c mice (20 mice/group) were primed with 100 µg of DNA-PyCSP. On week 6, the mice were boosted with 1 × 109 pu of HuAd5-PyCSP, GC44-PyCSP, GC45-PyCSP or GC46-PyCSP (protection study 1), or 1 × 109 pu of HuAd5-PyCSP or GC46-PyCSP (protection studies 2 and 3). Five days before (study day 38) and 9 days after (study day 52) the adenovirus boost, the mice were bled and sera isolated for antibody studies. Twelve days after the boost, 6 mice/group were euthanized and splenocytes isolated for T cell studies. On week 8, 14 mice/group were challenged with 100 P. yoelii sporozoites. Blood stage parasitaemia was evaluated 5–14 days post-challenge