Fig. 1

Infectivity of gametocyte-infected whole blood samples after different concentration methods. a The starting material of gametocyte-infected whole blood was 300 µL for the controls, which was fed to mosquitoes directly. For all non-control feeds, starting material was 1500 µL of the same gametocyte-infected whole blood. Gametocytes from this starting material were concentrated, eluted and reconstituted in 400 µL of uninfected blood, from which 300 µL was fed to the mosquitoes. Vertical bars indicate mean with SEM. **p < 0.01, ***p < 0.001. b Gametocyte density per sample from feeding material measured by Pfs25 qRT-PCR from experiments 1 and 2