Fig. 4

Protocol for automated counting of Plasmodium infection rate. After hydrophilic treatment of COC plates (a), parasite-infected RBCs stained with SYTO21 were added to the plate with various sizes of plastic frames (b). A picture of a plate is also shown (c). Cells were allowed to bind to the plate. After removing non-adherent cells, the cells were automatically counted using an inverted fluorescence microscope and MetaMorph Offline software