The implementation of long-lasting insecticidal bed nets has differential effects on the genetic structure of the African malaria vectors in the Anopheles gambiae complex in Dielmo, Senegal

Table 1 Sampling and identification of mosquitoes

Protocol of identification	Years	Months	Location of capture	An. coluzzii	An. gambiae	Hybrids	An. arabiensis	Total
A (n = 611)	2006	September	Indoor	21	14	1	7	43
		September	Outdoor	44	4	0	17	65
		October	Indoor	10	6	3	6	25
		October	Outdoor	18	5	0	9	32
		Total		93	29	4 (3.2%)	39	165
	2008	July	Indoor	0	2	0	53	55
		July	Outdoor	1	0	0	82	83
		October	Indoor	14	3	1	35	53
		October	Outdoor	10	2	0	24	36
		Total		25	7	1 (3.03%)	194	227
	2010	September	Indoor	8	20	1	42	71
		September	Outdoor	7	13	0	31	51
		October	Indoor	1	16	0	19	36
		October	Outdoor	2	16	0	43	61
		Total		18	65	1 (1.2%)	135	219
	Total			136	101	6	368	611
B (n = 497)		An coluzzii		109	2	0	1	112 (97.32)
		An. gambiae		4	86	0	0	90 (95.55)
		An. coluzzii/An. gambiae hybrids		5	0	1	0	6 (16.66)
		An. arabiensis		0	1	0	288	289 (99.65)

A. Information on population size for each species identified each year, month and location of collection. The mosquitoes were identified according to Wilkins et al.’s protocol [47] (see “Methods”). B. A subset of individuals was also examined according to Santolamazza et al.’s protocol [49] (the percentage of congruent determination with the Wilkins et al.’s protocol is given in brackets)

ISSN: 1475-2875