Fig. 1

A single dose of rapamycin rescues mice from late-stage ECM. a Body weights and food intake of female C57BL6 mice infected with Plasmodium berghei ANKA on day 0 and injected with vehicle or rapamycin (5 or 25 mg/kg) on day 4 (arrow) post-infection as indicated. b Circulating cytokines over the course of infection in female C57BL6 mice infected with P. berghei ANKA on day 0 (n = 4/time point). c Time course of infection-induced brain vasculature permeability. C57BL6 mice (3 per day) were infected with P. berghei ANKA that expresses mCherry (Pb-mCherry). On the indicated day, mice were injected with FITC-Dextran to analyze intravascular diffusion and extracellular permeability using intra-vital microscopy. Upper panels are composite images showing FITC-mCherry expression. The lower panels are representative images of the FITC channel, displayed as heat-map LUTs for quantitation. Evaluation of intravascular and extravascular—diffused-FITC-dextran mean fluorescence intensity (MFI) is quantified and plotted at right. d Survival and peripheral parasitaemia in female C57BL6 mice infected on day 0 and injected with vehicle or rapamycin (5 or 25 mg/kg) on day 4 (arrow) post-infection as indicated (n = 5/group). e Survival (n = 9/group) and peripheral parasitaemia (n = 4/group) of mice infected on day 0 and treated on day 5 or 6 post-infection with vehicle alone or with rapamycin (25 mg/kg) as indicated. Values are mean ± SEM