Fig. 5

Rapamycin modulaes endothelial activation in the brain. a Representative immunohistochemical images of day 6 brains from vehicle or rapamycin treated mice, stained for the endothelial marker CD31 (green) and ICAM-1 (red). Bar graph next to the micrographs shows the quantitation of the ICAM-1 mean fluorescence intensity (MFI) of over 20 fields of view, each one consisting of multiple optical sections 30 μm deep. Scale bar = 50 μm. b Dot plots showing the distribution and surface area representing CD31/ICAM-1 co-localization in endothelial cells by microscopy. Color intensity scale represents the frequency of events within the distribution. Bar graph next to plots show the quantitation of the surface area presenting ICAM-1 co-localization on CD31 brain endothelial cells. c Relative gene expression of chemokines and endothelial activation markers on day 6 of infection in brains of mice (n = 5/group) treated with vehicle (V) or rapamycin (R, 5 mg/kg) on day 4. Gene expression was analyzed using the ^∆∆CT equation, and fold change is expressed respect to the vehicle treated group. Data represent mean ± SEM