Table 2 Primer sequences used for relative quantification of genes linked to the insecticide resistance phenotype in Anopheles funestus
Gene | Sequence (5′–3′) | Primer conc. ( μM) | Annealing temperature ( °C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
CYP6P9b F CYP6P9b R | CAG CGC GTA CAC CAG ATT GTG TAA TTA CAC CTT TTC TAC CTT CAA GTA ATT ACC CGC | 3 | 60 | 97 | [41] |
CYP6M7 F CYP6M7 R | CGT TGT ATG AGC TGG CGT TA GTG CAT CTC CAT GAC AGC AT | 3 | 60 | 116 | [41] |
RPL19 F RPL19 R | CCA ACT CGC GAC AAA ACA TTC ACC GGC TTC TTG ATG ATC AGA | 3 | – | 61 | [20] |
RPS7 F RPS7 R | TTA CTG CTG TGT ACG ATG CC GAT GGT GGT CTG CTG GTT C | 3 | – | 135 | [84] |