Fig. 2

Yeast interfering RNA larvicides induce significant mortality. Significant larval death was observed in larvae fed with gel-coated live (a) or heat-inactivated (b) yeast engineered to express shRNA corresponding to the Sac1.1 and otk.16 siRNA target sequences (as compared to control yeast expressing shRNA corresponding to the control siRNA sequence). Data were compiled from replicate experiments, with 120 larvae evaluated per condition for live yeast and 180 larvae assessed per condition for inactive yeast (see “Methods” for details). Data were statistically assessed by ANOVA with Tukey’s multiple comparison test. No statistically significant differences were observed in the larvicidal capacity of live vs. heat-inactivated gel-coated yeast interfering RNA larvicides. ***p < 0.001 in comparison to control-fed larvae; error bars denote SEMs