Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays

Table 1 Selected P. falciparum gene targets and primers of SYBRGreen RTqPCR assays

Gene ID	Gene alias	Chromosome	Forward primer	Reverse primer
PF3D7_1031000	pfs25	10	TGGAAATCCCGTTTCATACGC	ACCGTTACCACAAGTTACATTCT
PF3D7_1351600	pfGK	13	AAGTTGTATATTCCACATGCGGTT	TATGCACCCAGATGGAGATCTGATG
PF3D7_1311100	pf13	13	AGAACGAATATGCTCGAGAACGAATTAGAAA	TGCCTTTTCATCTGACACGT
PF3D7_0209000	pfs230p	2	CCCAACTAATCGAAGGGATGAA (Schneider et al. [15])	TGTTGTTCGATTCCAGTTGGT

The table shows the gene ID, gene alias, chromosome, forward and reverse primer sequences for amplification of female (pfs25 and pfGK) and male (pf13 and pfs230p) gametocyte-specific P. falciparum transcripts

ISSN: 1475-2875