Fig. 2

CPS-CQ immunized DRAGA mice elicit human cellular responses to the Pfspz and to the iRBCs. a Splenic cells (left) and liver mononuclear cells (right) from immunized DRAGA mice (n = 7) as in Fig. 1, were stimulated for 4 days with Pfspz or with iRBCs (see methods) and human cytokines secreted in cell culture supernatants were measured by Luminex. Control cultures were unstimulated (nil) or stimulated with un-infected RBCS (uRBCs). Bars represent secretion of IL-2, TNF, IFNγ, and IL-5 in individual mice. While splenic and liver mononuclear cells responded to stimulation with Pfspz, only the liver cells responded to stimulation with iRBCs. b Spearman correlation for the splenic and liver total human cytokine responses to Pfspz (upper) and for each of the individual human cytokines (IL-2, TNF, IFNγ, IL-5) (lower). c Spearman correlation for the total human cytokine responses to the Pfspz and iRBCs within the liver (upper) and for each of the individual human cytokines (IL-2, TNF, IFNγ, IL-5) (lower)