Fig. 4
From: Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
In vivo anti-malarial activity of methylene blue against liver and blood stage. Mice were infected with 10,000 sporozoites. MB (50 and 100 mg/kg) and PQ (50 mg/kg) were administreted on day − 1, 0 and 1 to monitor activity against liver stage on day 2 and on day 3 and 4 to monitor blood stage activity. a Representative in vivo images (IVIS) of luminescence show in the liver of live Balb/C mice at different time points. Rainbow images show the relative levels of luminescence ranging from low (blue), to medium (green), to high (yellow/red). b Mean luminescence levels (photons/sec) of each group at day 2, 3 and 4; (**p = 0.0031, ***p = 0.0001, ****p < 0.0001). c Blood stage patency was monitored in mice by Giemsa-stained blood smears on day 3, 4 and 6. The results are expressed as the mean ± standard errors of the mean of 4 or 5 measurements