Differential plasma microvesicle and brain profiles of microRNA in experimental cerebral malaria

Malaria Journal

Table 1 Choice of targets for validation by RT-qPCR from OpenArray analysis

miRNA	Significance of fold change in CM vs. NI MV
miRNA	OpenArray	RT-qPCR
hsa-miR-328	− 2.5* ± 0.93	Not tested^a
hsa-miR-335*	− 3.0* ± 1.13	Not tested^a
mmu-miR-16*	2.8** ± 0.65	Not tested^a
mmu-miR-21*	5.0** ± 0.88	Not tested^a
mmu-miR-297a*	5.8* ± 1.60	Not tested^a
mmu-miR-685	3.0* ± 1.00	Not tested^a
mmu-miR-1949	5.0* ± 1.69	Not tested^a
hsa-miR-590-5p	Unique to NI	Not validated^b
rno-miR-450	Unique to CM	Not validated^b
mmu-miR-10b	2.7* ± 0.85	Not validated^b
hsa-miR-146a	3.2** ± 0.68	7.2* ± 2.74
hsa-miR-150	1.8* ± 0.64	2.7 (ns) ± 2.26
hsa-miR-205	2.3* ± 0.75	− 0.5 (ns) ± 1.89
hsa-miR-486	2.3*** ± 0.18	4.7 (ns) ± 1.45
mmu-miR-193b	− 2.7** ± 0.62	− 7.5* ± 0 62
mmu-miR-215	2.1* ± 0.55	4.6 (ns) ± 99.39^c
mmu-miR-467a	− 2.0* ± 0.69	− 5.6 (ns) ± 0.96

The list of significantly differentially expressed miRNA in CM vs NI MV from the OpenArray analysis was compared with the results obtained by RT-qPCR analysis. Results presented as relative quantification ± SEM
P: * = 0.05–0.01, ** = 0.01–0.0001, *** ≤ 0.0001
^amiRNA not validated by RT-qPCR due to lack of relevance to this study
^bmiRNA not validated despite being tested by RT-qPCR due to technical issues
^cLarge SEM variation due to outliers in NI and CM MV groups

ISSN: 1475-2875