Fig. 6

a Confocal micrographs of immobilized P. falciparum infected red blood cells stained with DAPI and either FITC-tagged aptamers or rPfLDH-specific antibodies. b Quantification of FITC-tagged aptamers and rPfLDH-specific antibody fluorescence associated with immobilized P. falciparum infected red blood cells (n = 12; p ≤ 0.05). FITC tagged aptamers included LDHp 1, LDHp 11, rLDH 4, rLDH 7, pL1 and concatemer, C7. IgY antibodies against rPfLDH were detected with FITC-labeled secondary antibody. Left to right: phase-contrast; green channel (FITC; excitation = 490; emission = 525 nm); blue channel (DAPI; excitation = 360 nm; emission = 460 nm), and merged blue- and green-channel images. Each scale bar represents 2 µm. *Significant: p = 0.01–0.05 (Dunn’s multiple comparison test). **Very significant: p = 0.001–0.01 (Dunn’s multiple comparison test). ***Extremely significant: p < 0.001 (Dunn’s multiple comparison test)