Development of a high-throughput flexible quantitative suspension array assay for IgG against multiple Plasmodium falciparum antigens

Table 1 Mean coefficient of variation (CV) and standard deviation (SD)

Analyte	CV between repeats (%) log₁₀ MFI (3 repeats) Intra-assay		CV between days (%) log₁₀ MFI (n = 22) Inter-assay		CV between days (%) AU/mL (n = 22) Inter-assay
Analyte	CV	SD	CV	SD	CV	SD
AMA-1 3D7	2.31	± 4.47	4.35	± 4.07	25.79	± 12.16
AMA-1 FVO	4.96	± 16.59	7.57	± 9.76	28.65	± 14.7
EBA-175	1.47	± 2.79	4.59	± 3.16	35.25	± 33.16
CSP	2.29	± 7.92	7.97	± 9.61	27.73	± 11.46
LSA-1	11.05	± 18.43	13.21	± 8.12	43.27	± 17.81
Control BSA	22.54	± 23.74	21.38	± 7.08	64.12	± 31.18
Overall	7.66	± 15.89	9.24	± 8.98	31.28	± 22.74

Between triplicates and days of analysis in log₁₀MFI or AU/mL for each antigen of the multiplex panel

ISSN: 1475-2875