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Table 2 Cycling conditions for amplification of the PCR primary and secondary round

From: Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination

PCR conditions
Primary round PCR programme: 3 h Nested round PCR programme: 3 h
1. Initial denaturation—94 °C × 5 m 1. Initial denaturation—94 °C × 5 m
2. 40 Cycles—94 °C × 30 s, 52.5 °C × 90 s, 68 °C × 90 s 2. 40 Cycles—94 °C × 30 s, 60 °C × 90 s, 72 °C × 90 s
3. Final elongation—68 °C × 10 m 3. Final elongation—72 °C × 10 m
4. Hold at 4 °C 4. Hold at 4 °C