Fig. 1

Increasing equivalents of α-HRP2 antibodies reduced free HRP2 detectable by ELISA by forming immune complexes. Heating samples at 80 °C for 20 min dissociated these complexes, completely restoring ELISA signal, regardless of α-HRP2 excess
Increasing equivalents of α-HRP2 antibodies reduced free HRP2 detectable by ELISA by forming immune complexes. Heating samples at 80 °C for 20 min dissociated these complexes, completely restoring ELISA signal, regardless of α-HRP2 excess