Fig. 2From: Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral bloodPlasmodium falciparum 18S rRNA biomarker in rhesus macaques before and after high-dose PfSPZ inoculation. Tissue samples and whole blood were collected and preserved in lysis buffer as described. Nucleic acids were extracted and RT-PCR performed as described. The Plasmodium 18S rRNA biomarker was detected only from liver samples. Open circles, pre-inoculation; closed circles, day 3 post-inoculation; closed triangles, day 6 post-inoculation. Bars show mean ± standard deviation. Spleen was tested from only one uninfected animal, and lung samples were not tested from uninfected animals. Each data point represents a unique sample obtained from an individual animal at the stated time pointBack to article page