Fig. 4

Growth inhibitory activity (GIA) of various mAbs directed against rPfeno. a Effect of varying concentrations of H12E1, H12B8 and B1B3 mAbs on growth of P. falciparum 3D7. b Effect of varying concentrations of H12E1 IgG on P. falciparum 3D7 (chloroquine sensitive) and P. falciparum INDO (chloroquine resistant) strains. c Effect of exposure of the parasite cultures to H12E1 antibody on subsequent growth. Two sets of tightly synchronized ring stage parasites (~ 1%) were cultured in presence of varying amounts (0–60 µg/ml) of H12E1 IgG. At 48 h, parasitemia was measured for the first set of cultures while the second set of cultures was washed free of H12E1 antibody and supplemented with fresh medium. This was grown for another 48 h (in absence of mAb) and % parasitemia was determined at 96 h. Results are presented as % parasitemia at 0, 48 and 96 h for different concentrations of H12E1 and (d) % parasitemia vs [H12E1]. Values are presented as mean ± SD for triplicate samples. AU^#, arbitrary units; * Marks the samples that had significant growth inhibition as compared to the control samples (*p < 0.05, **p < 0.01, ***p < 0.001)