Optimization of a magnetic bead-based assay (MAGPIX®-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings

Table 1 Seroprevalence and IgG level of response

Antigen	Dielmo		Ndiop		Urban SM
Antigen	IgG level	Prev^c (%)	IgG levels	Prev (%)	IgG levels	Prev (%)
MSP4-p20^a	1103	64	2684	84	1315	69
[Range]	[28–10,580]	64	[42–13,160]	84	[23–6381]	69
PfCSP^a	262	52	536	57	187	47
[Range]	[22–985]	52	[22–4969]	57	[18–1061]	47
PfAMA1^a	282	52	402	49	456	58
[Range]	[12–2352]	52	[43–3065]	49	[46–8930]	58
PmCSP^a	691	56	537	62	79	19
[Range]	[28–7365]	56	[29–9278]	62	[23–991]	19
MSP4-p20^b	4.5	56	5.9	70	6.0	75
[Range]	[1–14.7]	56	[1–15.3]	70	[1–12.9]	75
Schizont Ag^b	3.5	64	3.1	76	2.2	36
[Range]	[1–9.3]	64	[1–6.0]	76	[1–7.4]	36

^a Levels and prevalence of Ab responses measured by MBA (expressed in MFI)
^b Levels and prevalence of Ab responses measured by ELISA (expressed in OD ratio)
^c Prev prevalence of responders

ISSN: 1475-2875