Deletions of pfhrp2 and pfhrp3 genes of Plasmodium falciparum from Honduras, Guatemala and Nicaragua

Fontecha, Gustavo; Mejía, Rosa E.; Banegas, Engels; Ade, Maria Paz; Mendoza, Lisandro; Ortiz, Bryan; Sabillón, Isaac; Alvarado, Gerardo; Matamoros, Gabriela; Pinto, Alejandra

doi:10.1186/s12936-018-2470-7

Malaria Journal

Table 1 Primer sequences and amplification conditions of pfhrp2 and pfhrp3 genes, and their respective flanking sequences

From: Deletions of pfhrp2 and pfhrp3 genes of Plasmodium falciparum from Honduras, Guatemala and Nicaragua

Target sequence	Reaction	Primer	Primer sequence	Annealing temp (°C)	Amplicon size (bp)
pfhrp2 UPSTREAM PF3D7_0831900, (MAL7P1.230)	Primary	230F1	5′GATATCATTAGAAAACAAGAGCTTAG3′	63	301
	Primary	230R	5′TATCCAATCCTTCCTTTGCAACACC3′	63	301
	Semi-nested	230F	5′TATGAACGCAATTTAAGTGAGGCAG3′	65
	Semi-nested	230R	5′TATCCAATCCTTCCTTTGCAACACC3′	65
pfhrp2 Exon 1–2, PF3D7_0831800	Primary	2E12F1	5′GGTTTCCTTCTCAAAAAATAAAG3′	55	228
	Primary	2E12R1	5′TCTACATGTGCTTGAGTTTCG3′	55	228
	Nested	2E12F	5′GTATTATCCGCTGCCGTTTTTGCC3′	62
	Nested	2E12R	5′CTACACAAGTTATTATTAAATGCGGAA3′	62
pfhrp2 DOWNSTREAM PF3D7_0831700, (MAL7P1.228)	Primary	228F	5′AGACAAGCTACCAAAGATGCAGGTG3′	60	198
	Primary	228R	5′TAAATGTGTATCTCCTGAGGTAGC3′	60	198
	Semi-nested	228F1	5′CCATTGCTGGTTTAAATGTTTTAAG3′	63
	Semi-nested	228R	5′TAAATGTGTATCTCCTGAGGTAGC3′	63
pfhrp3 DOWNSTREAM PF3D7_1372100, (MAL13P1.485)	Primary	485F	5′TTGAGTGCAATGATGAGTGGAG3′	60	241
	Primary	485R	5′AAATCATTTCCTTTTACACTAGTGC3′	60	241
	Semi-nested	485F1	5′GTTACTACATTAGTGATGCATTC3′	59
	Semi-nested	485R	5′AAATCATTTCCTTTTACACTAGTGC3′	59
pfhrp3 Exon 1–2, PF3D7_1372200	Primary	3E12F1	5′GGTTTCCTTCTCAAAAAATAAAA3′	53	225
	Primary	3E12R1	5′CCTGCATGTGCTTGACTTTA3′	53	225
	Nested	3E12F	5′ATATTATCGCTGCCGTTTTTGCT3′	62
	Nested	3E12R	5′CTAAACAAGTTATTGTTAAATTCGGAG3′	62
pfhrp3 UPSTREAM PF3D7_1372400, (MAL13P1.475)	Primary	475F	5′TTCATGAGTAGATGTCCTAGGAG3′	55	212
	Primary	475R	5′TCGTACAATTCATCATACTCACC3′	55	212
	Semi-nested	475F	5′TTCATGAGTAGATGTCCTAGGAG3′	61
	Semi-nested	475R1	5′GGATGTTTCGACATTTTCGTCG3′	61
pfhrp2 Exon 2	Primary	Pfhrp2F1	5′CAAAAGGACTTAATTTAAATAAGAG3′	55	600–950
	Primary	Pfhrp2R1	5′AATAAATTTAATGGCGTAGGCA3′	55	600–950
	Semi-nested	Pfhrp2F2	5′ATTATTACACGAAACTCAAGCAC3′	55
	Semi-nested	Pfhrp2R1	5′AATAAATTTAATGGCGTAGGCA3′	55
pfhrp3 Exon 2	Primary	Pfhrp3F1	5′AATGCAAAAGGACTTAATTC3′	55	600–950
	Primary	Pfhrp3R1	5′TGGTGTAAGTGATGCGTAGT3′	55	600–950
	Semi-nested	Pfhrp3F2	5′AAATAAGAGATTATTACACGAAAG3′	55
	Semi-nested	Pfhrp3R1	5′TGGTGTAAGTGATGCGTAGT3′	55

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ISSN: 1475-2875

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