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Table 1 Primer sequences and amplification conditions of pfhrp2 and pfhrp3 genes, and their respective flanking sequences

From: Deletions of pfhrp2 and pfhrp3 genes of Plasmodium falciparum from Honduras, Guatemala and Nicaragua

Target sequence Reaction Primer Primer sequence Annealing temp (°C) Amplicon size (bp)
pfhrp2 UPSTREAM PF3D7_0831900, (MAL7P1.230) Primary 230F1 5′GATATCATTAGAAAACAAGAGCTTAG3′ 63 301
230R 5′TATCCAATCCTTCCTTTGCAACACC3′
Semi-nested 230F 5′TATGAACGCAATTTAAGTGAGGCAG3′ 65  
230R 5′TATCCAATCCTTCCTTTGCAACACC3′
pfhrp2 Exon 1–2, PF3D7_0831800 Primary 2E12F1 5′GGTTTCCTTCTCAAAAAATAAAG3′ 55 228
2E12R1 5′TCTACATGTGCTTGAGTTTCG3′
Nested 2E12F 5′GTATTATCCGCTGCCGTTTTTGCC3′ 62  
2E12R 5′CTACACAAGTTATTATTAAATGCGGAA3′
pfhrp2 DOWNSTREAM PF3D7_0831700, (MAL7P1.228) Primary 228F 5′AGACAAGCTACCAAAGATGCAGGTG3′ 60 198
228R 5′TAAATGTGTATCTCCTGAGGTAGC3′
Semi-nested 228F1 5′CCATTGCTGGTTTAAATGTTTTAAG3′ 63  
228R 5′TAAATGTGTATCTCCTGAGGTAGC3′
pfhrp3 DOWNSTREAM PF3D7_1372100, (MAL13P1.485) Primary 485F 5′TTGAGTGCAATGATGAGTGGAG3′ 60 241
485R 5′AAATCATTTCCTTTTACACTAGTGC3′
Semi-nested 485F1 5′GTTACTACATTAGTGATGCATTC3′ 59  
485R 5′AAATCATTTCCTTTTACACTAGTGC3′
pfhrp3 Exon 1–2, PF3D7_1372200 Primary 3E12F1 5′GGTTTCCTTCTCAAAAAATAAAA3′ 53 225
3E12R1 5′CCTGCATGTGCTTGACTTTA3′
Nested 3E12F 5′ATATTATCGCTGCCGTTTTTGCT3′ 62  
3E12R 5′CTAAACAAGTTATTGTTAAATTCGGAG3′
pfhrp3 UPSTREAM PF3D7_1372400, (MAL13P1.475) Primary 475F 5′TTCATGAGTAGATGTCCTAGGAG3′ 55 212
475R 5′TCGTACAATTCATCATACTCACC3′
Semi-nested 475F 5′TTCATGAGTAGATGTCCTAGGAG3′ 61  
475R1 5′GGATGTTTCGACATTTTCGTCG3′
pfhrp2 Exon 2 Primary Pfhrp2F1 5′CAAAAGGACTTAATTTAAATAAGAG3′ 55 600–950
Pfhrp2R1 5′AATAAATTTAATGGCGTAGGCA3′
Semi-nested Pfhrp2F2 5′ATTATTACACGAAACTCAAGCAC3′ 55  
Pfhrp2R1 5′AATAAATTTAATGGCGTAGGCA3′
pfhrp3 Exon 2 Primary Pfhrp3F1 5′AATGCAAAAGGACTTAATTC3′ 55 600–950
Pfhrp3R1 5′TGGTGTAAGTGATGCGTAGT3′
Semi-nested Pfhrp3F2 5′AAATAAGAGATTATTACACGAAAG3′ 55  
Pfhrp3R1 5′TGGTGTAAGTGATGCGTAGT3′