Prevalence of asymptomatic Plasmodium infections with sub-microscopic parasite densities in the northwestern border of Thailand: a potential threat to malaria elimination

Table 2 Detailed comparison of LAMP and nested PCR combined with restriction enzyme digestion for Plasmodium identifications

Parasite(s) detected by each method^a (no. of samples; n = 3650 blood samples)
LAMP	Nested PCR combined with RE digestion of LAMP products^b
P. falciparum (27)	P. falciparum (26), negative (1)^c
P. vivax (47)	P. vivax (45), negative (2)^d
P. falciparum + P. vivax (4)	P. falciparum + P. vivax (4)
P. malariae (2)	P. malariae (2)
Plasmodium spp.^e (8)	Plasmodium spp. (8)
Negative (3562)	Negative (3562)

^aEach row displays the results obtained from identical blood samples. Discordant results between LAMP and nested PCR combined with restriction enzyme digestion are showed in italicface
^bGenus and species-specific LAMP products were digested with restriction enzyme to confirm the specificity of LAMP products
^cPositive for P. falciparum by LAMP but negative after restriction enzyme digestion of LAMP products
^dPositive for P. vivax by LAMP but negative after restriction enzyme digestion of LAMP products
^ePositive for genus-specific LAMP but negative for species-specific LAMP

ISSN: 1475-2875