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Fig. 6 | Malaria Journal

Fig. 6

From: Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library

Fig. 6

Expression and purification of recombinant cytosolic H8VNAR using pET-28a vector. Proteins were separated on 15% SDS-PAGE and stained with Coomassie Blue (left panel). H8VNAR was detected by Western Blot with anti-His tag antibody and a ~ 14 kDa band clearly apparent. Lane 1 represents total protein from non IPTG-induced bacteria cells; lane 2 represents total protein from IPTG-induced bacteria cells; and lane 3 represents purified cytosolic H8VNAR protein

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