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Fig. 7 | Malaria Journal

Fig. 7

From: Isolation and characterization of malaria PfHRP2 specific VNAR antibody fragments from immunized shark phage display library

Fig. 7

Expression and purification of recombinant periplasmic DsbA-H8VNAR using pDSB-28Y vector. Proteins were separated on 15% SDS-PAGE and stained with Coomassie blue (left panel). DsbA-H8VNAR was detected by Western Blot with anti-His tag antibody and a ~ 14 kDa band clearly apparent. Lane 1 represents total protein from non IPTG-induced bacteria cells; lane 2 represents total protein from IPTG-induced bacteria cells; and lane 3 represents purified periplasmic DsbA-H8VNAR protein

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