Table 1 Primers used for shark VNAR library construction and sequencing
Primers description | Nucleotide sequence (5′→3′) | Applications |
|---|---|---|
Leader-F | ATGAATATTTTCTTGCYGTCAGTCC | 1st PCR |
C-DomainR | CCTCTCTGTTCTTCRGTTGCAGAGT | 1st PCR |
FR1F1 | RCAWGGGTRGACCAAACACC | Nested PCR |
FR4R | TTTCACGGTYARTRCGGTGCC | Nested PCR |
T7SelectUP (For) | GGAGCTGTCGTATTCCAGTC | T7 Phage sequencing |
T7SelectDOWN (Rev) | AACCCCTCAAGACCCGTTTA | T7 Phage sequencing |
T7Seq_For | TAATACGACTCACTATAGGG | pET vector sequencing forward |
T7Seq_Rev | CTAGTTATTGCTCAGCGGTG | pET vector sequencing reverse |