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Fig. 4 | Malaria Journal

Fig. 4

From: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Fig. 4

Antibody competition titration assays using MSP119 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP119 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP119; b P. malariae MSP119; c P. ovale MSP119; d P. vivax MSP119. Multiplex bead assays were performed as described in “Methods” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

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