Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Table 1 Impact of bead complexity on multiplex bead assay response values using beads coated with MSP1₁₉ proteins from four malaria species

Sample	Assay type	Pf¹ MSP1₁₉ (MFI-bg)	Pm MSP1₁₉ (MFI-bg)	Po MSP1₁₉ (MFI-bg)	Pv MSP1₁₉ (MFI-bg)	Pf CSP-peptide (MFI-bg)	GST (MFI-bg)
Pan Plasmodium Lot 8 serum	Individual monoplex	28,537	355	721	23,449	N/A	N/A
Pan Plasmodium Lot 8 serum	Multiplex	28,442	351	724	23,688	2281	41
P. malariae Lot 2 serum	Individual monoplex	19	10,413	6	2	N/A	N/A
P. malariae Lot 2 serum	Multiplex	20	10,878	6	2	170	11
Mozambique donor 15	Individual monoplex	28,088	1437	16,504	260	N/A	N/A
Mozambique donor 15	Multiplex	28,242	1446	17,352	272	26,039	53
Mozambique donor 7	Individual monoplex	9396	734	9566	78	N/A	N/A
Mozambique donor 7	Multiplex	9484	798	10,623	86	22,359	11
Mozambique donor 8	Individual monoplex	28,034	23,220	11	45	N/A	N/A
Mozambique donor 8	Multiplex	28,438	23,378	14	47	15,819	11
Mozambique elution mix	Individual monoplex	28,842	27,229	20,193	24,113	N/A	N/A
Mozambique elution mix	Multiplex	28,911	27,082	21,585	24,048	17,682	279

ISSN: 1475-2875