Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

Table 7 IgG sub-class and antibody avidity index for samples from Mozambique

Sample	Antibody detected	Description	Pf MSP1₁₉ (MFI-bg)	Pm MSP1₁₉ (MFI-bg)	Po MSP1₁₉ (MFI-bg)	Pv MSP1₁₉ (MFI-bg)	PfCSP (MFI-bg)	GST (MFI-bg)	Tet (MFI-bg)
Mozambique 13	IgG₁	No treatment	25,380	7236	369	23,068	7081	1	700
	IgG₂	No treatment	1255	214	36	778	21,283	3	17
	IgG₃	No treatment	404	441	50	281	7555	0	10
	IgG₄	No treatment	26	4	3	9	0	0	28
	Total IgG	No treatment	31,255	26,542	1198	30,077	28,809	12	1861
	Total IgG	6 M urea wash	31,009	5724	623	2297	28,005	3	2253
	Total IgG	Avidity index^a	0.99	0.22	0.52	0.08	0.97	N/D	1.21
Mozambique 16	IgG₁	No treatment	2425	627	89	190	3722	9	5100
	IgG₂	No treatment	107	41	6003	22,534	205	4	168
	IgG₃	No treatment	30,533	3401	43	55	5654	2	107
	IgG₄	No treatment	52	6	7	7	13	0	1206
	Total IgG	No treatment	30,369	59,12	16,287	23,777	20,145	35	24,367
	Total IgG	6 M Urea wash	29,850	3456	1689	223	7332	4	24,683
	Total IgG	Avidity index	0.98	0.58	0.10	0.01	0.36	N/D	1.01
Mozambique 15	IgG₁	No treatment	14,698	416	4196	89	11,752	1	2348
	IgG₂	No treatment	573	18	58	26	578	4	67
	IgG₃	No treatment	30,026	2400	48	64	19,941	0	86
	IgG₄	No treatment	3271	9	4	3	281	0	2934
	Total IgG	No treatment	30,749	1652	18,046	302	28,269	8	14,066
	Total IgG	6 M Urea wash	30,298	1183	6032	35	25,232	2	13,336
	Total IgG	Avidity index	0.99	0.72	0.33	0.12	0.89	N/D	0.95
Mozambique 12	IgG₁	No treatment	269	45	35	57	7043	2	4212
	IgG₂	No treatment	28	15	26	15	468	5	188
	IgG₃	No treatment	24,515	11,988	8263	8259	22,011	3	33
	IgG₄	No treatment	6	1	2	3	9	0	177
	Total IgG	No treatment	23,607	6741	4437	4162	26,444	16	21,638
	Total IgG	6 M Urea wash	719	36	29	61	19,765	4	19,375
	Total IgG	Avidity index	0.03	0.01	0.01	0.01	0.75	N/D	0.90

^aThe total IgG antibody avidity index, indicated in italic cells, was calculated by dividing the total IgG response after 6 M urea wash by the total IgG response with no treatment
N/D not determined

ISSN: 1475-2875