Fig. 1
Limit of detection and variation of the gametocyte multiplex assay. Individual results of 8 technical replicates for female-sorted (a) and male-sorted (b) gametocytes of decreasing densities. Seven measurements (dots) obtained by calculating the reference from one randomly chosen dilution series. The coefficient of variation (filled bars) was calculated as standard deviation/mean of the respective calculated densities. Sample positivity in n = 26 independent experiments is indicated in the white boxes