Table 1 Primer and probe sequences for qRT-PCR assays, with references for earlier reported methods and primers
Gene ID Name | Fwd primer seq Rev primer seq | Probe seq | Fluoro-phore |
|---|---|---|---|
PF3D7_1031000 Pfs25 [36] | GAAATCCCGTTTCATACGCTTG AGTTTTAACAGGATTGCTTGTATCTAA | – | – |
PF3D7_1031000 Pfs25 [34] | Â | 6FAM-ccgtttcatacgcttgtaa-MGB | FAM |
PF3D7_0903800 CCp4 (MPX) | CACATGAATATGAGAATAAAATTG* TAGGCGAACATGTGGAAAG | AGCAACAACGGTATGTGCCTTAAAACG | Texas Red |
PF3D7_0903800 CCp4 (qRT-PCR) | CACATGAATATGAGAATAAAATTG* TAGGCGAACATGTGGAAAG | – | – |
PF3D7_1469900 PfMGET (MPX) | CGGTCCAAATATAAAATCCTG* TGTGTAACGTATGATTCATTTTC | CAGCTCCAGCATTAAAAACAC | FAM |
PF3D7_1469900 PfMGET (qRT-PCR, [28]) | CGGTCCAAATATAAAATCCTG* GTGTTTTTAATGCTGGAGCTG | – | – |