Skip to main content

Table 1 Genotypes and their frequencies of propeller domain in the k13 gene from P. falciparum isolates of Yunnan Province and Myanmar

From: Genetic association between the Pfk13 gene mutation and artemisinin resistance phenotype in Plasmodium falciparum isolates from Yunnan Province, China

Genotypes and/or gene mutation loci HaplotypesC Coding single nucleotide polymorphism Locus code and base substitutionD Total number (%) 1. Malaria cases in Yunnan Province No. (%) 2. Malaria cases in Myanmar No. (%)
Sequences of k13 gene    384 194 190
Wild genotype Hap_1 202 126 76
Synonymous mutations      
 G449G Hap_17 c.1347 T > G GGT > GGG 1 (0.3) 0 1 (0.5)
 T451T Hap_13 c.1353 T > C TTT > TTC 1 (0.3) 1 (0.5) 0
 G674G Hap_22 c.2022 G > A GGG > GGA 1 (0.3) 0 1 (0.5)
Single mutants genotype      
 F446I* Hap_2 c.1338 T > A TTT > ATT 130 (33.6) 49 (25.3) 81 (42.6)
 G450V* Hap_15 c.1349 G > T GGA > GTA 1 (0.3) 0 1 (0.5)
 N458Y* Hap_7 c.1372 A > T AAT > TAT 3 (0.8) 2 (1.0) 1 (0.5)
 S459L* Hap_9 c.1376 C > T TCG > TTG 2 (0.5) 2 (1.0) 0
 C469Y* Hap_10 c.1407 G > A TGC > TAC 4 (1.0) 1 (0.5) 3 (1.6)
 A481V* Hap_20 c.1442 C > T GCT > GTT 2 (0.5) 0 2 (1.0)
 F483S* Hap_18 c.1448 T > C TTT > TCT 1 (0.3) 0 1 (0.5)
 L492S* Hap_19 c.1475 T > C TTA > TCA 1 (0.3) 0 1 (0.5)
 G533A* Hap_12 c.1598 G > C GGT > GCT 2 (0.5) 1 (0.5) 1 (0.5)
 P553L* Hap_16 c.1658 C > T CCG > CTG 3 (0.8) 0 3 (1.6)
 E556D* Hap_5 c.1668 A > T GAA > GAT 3 (0.8) 1 (0.5) 2 (1.0)
 P574L* Hap_8 c.1721 C > T CCT > CTT 7 (1.8) 2 (1.1) 5 (2.6)
 A578S* Hap_11 c.1732 G > T GCT > TCT 1 (0.3) 1 (0.5) 0
 C580Y* Hap_14 c.1739 G > A TGT > TAT 4 (1.0) 0 4 (2.1)
 V581I* Hap_3 c.1741 G > A GTT > ATT 1 (0.3) 1 (0.5) 0
 E668D* Hap_4 c.2004 G > C GAG > GAC 1 (0.3) 1 (0.5) 0
 A675V* Hap_23 c.2024 C > T GCT > GTT 1 (0.3) 0 1 (0.5)
 A676D* Hap_6 c.2027 C > A GCC > GAC 11 (2.9) 6 (3.2) 5 (2.6)
Double mutants genotype   
 Y519K* Hap_21 c.1855 T > A, c.1857 T > G TAT > AAG 1 (0.3) 0 1 (0.5)
 G449G/A676D* Hap_17 c.1337T > G, c.2027 C > A GGT > GGG,GCC > GAC Number was the same above synonymous mutations   Yes
Haplotype     23 12 18
Ka/Ks     12.2 16.3 10.9
He     0.6124 0.0481 0.044
H     0.0283 0.5182 0.651
Fst (P value)     0.0410A (0.0020B)  
  1. * Letters in bold indicate mutated amino acids; A: Group contribution ratio between group 1 and group 2 of Fst; B: P<0.05; C: The names of Haplotypes were the same of Fig. 4; D: The substituted bases highlighted with italic and bold in every code