Fig. 4

Transgenic Anopheles stephensi expressing Anopheles gambiae Saglin in the distal lateral lobes of the salivary glands of mosquitoes. a DNA sequence flanking the terminal inverted repeats (arrows) of the three piggyBac vectors present in the transgenic line used in this study. b Phase contrast and fluorescence images of salivary glands from non-transgenic females prepared for indirect immunofluorescence to localize An. gambiae Saglin using mAb2A3 as the primary antibody and Alexafluor 555-labeled goat-anti-mouse secondary antibody. No Saglin was detectable. c Phase contrast and fluorescence images of salivary glands from transgenic females prepared in parallel with those shown in b. Strong fluorescence was detected only in the distal lateral lobes of the salivary glands, as expected based on the known spatial patterns of expression of the AAPP promoter from An. stephensi that was used to regulate the expression of the An. gambiae Saglin transgene