Fig. 2

Analysis of S-glutathionylation on PfGluPho (a), PfG6PD (b), and PvG6PD (c). Pre-reduced enzymes were incubated with different GSSG concentrations for 10 min at 37 °C; potential S-glutathionylation was detected via western blot analysis using an anti-glutathione antibody. To study the reversibility of potential S-glutathionylation, reducing SDS-PAGE with DTT was performed. Samples of PfPrx1a were prepared in parallel as a positive control. Without DTT, PfPrx1a exists in a dimeric form; adding DTT shifts the conformation to a monomeric form. Ponceau staining of the membrane used for western blotting served as a loading control. Representative blots from at least two independent experiments are shown. Pos. ctrl.: positive control