Fig. 5

Evolution of parasite load predicted parasitaemia (PLPP) and flow cytometry measured parasitaemia in ICR-CD1 mice infected with 1 × 10⁵ luciferase-expressing P. berghei ANKA infected erythrocytes. Thirty ICR-CD1 female mice were infected IP with 1 × 10⁵ luciferase-expressing P. berghei infected erythrocytes. Data shown represents evolution of PLPP calculated using the linear and power Eqs. (2) and (3) as well as flow cytometry measured parasitaemia in all 30 mice on days 1, 2, 3, 4, 6, and 7 post infections. Each data point represents mean ± standard error for the PLPP and flow cytometry measured parasitaemia for 30, 30, 30, 30, 27 and 19 ICR-CD1 mice respectively on days 1, 2, 3, 4, 6, and 7 post infections. Three (3) and 8 (eight) ICR-CD1 mice had to be removed from the study respectively on days 6 and 7 post infections due to severe malaria symptoms. An unpaired t-test with Welch’s correction was used to compare differences between group means. A P-value < 0.05 was considered statistically significant (*P < 0.05)