Fig. 8

a LysRS family phylogenetic tree. Maximum Likelihood method was used to infer evolutionary history using Le_Gascuel_2008 model at 90% site coverage [150]. Phylogenetic tree calculations were done using MEGA7 [102]. The tree that had the highest log likelihood (-6116.25) is shown. Initial tree(s) for the heuristic search were obtained by using BioNJ and Neighbor-Join algorithms to a matrix of pairwise distances calculated using a JTT model, and then selecting the topology with higher log likelihood value. A Gamma distribution was used to calculate evolutionary rate differences among sites (5 categories (+G, parameter = 0.6075)). Eleven amino acid sequences were used for this analysis. There were 503 positions after the calculations. b LysRS pairwise sequence calculations. The sequence identity values of the sequences in the LysRS family is shown. The heatmap shows the identity scores as a colour-coded matrix for every aaRS sequence versus every aaRS sequence in this family. Conservation increases from blue colour to red colour in the heat map