Table 1 Guanidine thiocyanate (with alcohol) outperforms guanidine hydrochloride for extraction of DNA from dried blood spots
3M GuSCN | 3M GHCl | |||||
|---|---|---|---|---|---|---|
Alcohol | 0% | 16.7% ISOH | 16.7% ETOH | 0% | 16.7% ISOH | 16.7% ETOH |
Average Ct for Pf 18S rDNA (± SD) | Undetectable | 29.2 (± .1) | 29.3 (± .2) | Undetectable | Undetectable | Undetectable |
Average Ct for human actin (± SD) | 28.8 (± .2) | 27.3 (± .3) | 27.6 (± .3) | 29.6 (± .4) | Undetectable | Undetectable |