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Table 1 Guanidine thiocyanate (with alcohol) outperforms guanidine hydrochloride for extraction of DNA from dried blood spots

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

  3M GuSCN 3M GHCl
Alcohol 0% 16.7% ISOH 16.7% ETOH 0% 16.7% ISOH 16.7% ETOH
Average Ct for Pf 18S rDNA (± SD) Undetectable 29.2 (± .1) 29.3 (± .2) Undetectable Undetectable Undetectable
Average Ct for human actin (± SD) 28.8 (± .2) 27.3 (± .3) 27.6 (± .3) 29.6 (± .4) Undetectable Undetectable
  1. Experiments done with samples at parasite density of 2000 parasites/mL
  2. Pf, Plasmodium falciparum; GuSCN, guanidine thiocyanate; GHCl, guanidine hydrochloride; ISOH, isopropanol; ETOH, ethanol; SD, standard deviation; Ct, cycle threshold