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Table 6 The current protocol outperforms Qiagen QIAamp for purification of parasite DNA from dried blood spots

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

Method

Current protocol

Qiagen QIAamp

Filter paper

Whatman 3MM

Whatman

903 PS

Whatman 3MM

Whatman

903 PS

Average Ct for Pf 18S rDNA (± SD)

30.2 (± .1)

30.2 (± .2)

35.1 (± .1)

35.0 (± .1)

  1. Experiments done with samples at parasite density of 2000 parasites/mL
  2. Pf, Plasmodium falciparum; PS, protein saver; SD, standard deviation; Ct, cycle threshold
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Malaria Journal

ISSN: 1475-2875

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