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Table 6 The current protocol outperforms Qiagen QIAamp for purification of parasite DNA from dried blood spots

From: An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

Method Current protocol Qiagen QIAamp
Filter paper Whatman 3MM Whatman 903 PS Whatman 3MM Whatman 903 PS
Average Ct for Pf 18S rDNA (± SD) 30.2 (± .1) 30.2 (± .2) 35.1 (± .1) 35.0 (± .1)
  1. Experiments done with samples at parasite density of 2000 parasites/mL
  2. Pf, Plasmodium falciparum; PS, protein saver; SD, standard deviation; Ct, cycle threshold