Fig. 1

Consistent outcomes between 5 ml flask and 96-well plate methods in parasite competitive growth assays. Competition assays began with a parasite ratio of approximately 0.5:0.5. DNA microsatellite markers were used to track the competitive growth of parasites over time. Parasite competition P3 versus NHP4026 (n = 3) revealed the ability of NHP4026 to outcompete P3 by day 22 in both 5 ml flasks (a) and 96-well plates (b). The competition between P3 and P1 (n = 3) demonstrated the ability of P1 to outcompete P3 in 5 ml flasks by day 34 (c) and in 96-well plates by day 50 (d). Overall outcome results were consistent between 5 ml flask and 96-well plate, confirming the reliability of 96-well plate competitive growth assay methodology