Fig. 2

Win/loss records between parasite lines generate a fitness hierarchy. Win/loss records were determined for each parasite line and used to order lines from highest competitive fitness to lowest fitness. Arrows point from the winning (more fit) parasite to the losing (less fit) parasite. The win/loss record hierarchy ranked the kelch13-wt, slow clearance NHP4026 as having the highest fitness (7 wins, 0 losses: 7-0 record). Both NHP3032 and NHP4373 were unable to outcompete any other parasite, and the NHP3032 versus NHP4373 competition was unresolved, giving both isolates a record of 0 wins, 0 losses, 1 tie (0-0-1), suggesting potential fitness disadvantages in these isolates. NHP4302 had a record of 2-5. The hierarchy also shows that the kelch13 mutant isolates, NHP1337 (3-4 record), NHP4333 (4-3 record), and NHP4076 (5-2 record) have middle-range fitness and have a fitness cost relative to the uncharacterized resistance in NHP4026 and to the sensitive lab line, NF54 (6-1 record)