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Fig. 1 | Malaria Journal

Fig. 1

From: Harmonization study between three laboratories for expression of malaria vaccine clinical trial IgG antibody ELISA data in µg/mL

Fig. 1

Conversion factors determined using the harmonized method. Six total IgG samples from the VAC054 trial were divided amongst the three laboratories and used to determine conversion factors. AMA1-specific IgG was affinity-purified using the same protocol and FMP2.1 protein coupled to purification columns. The concentration of thus affinity-purified AMA1-specific IgG in ng/mL was plotted against ELISA AU obtained using in-house ELISA protocols with in-house AMA1 protein. The slope of each linear regression line was used as the “harmonized conversion factor” (CF)

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Malaria Journal

ISSN: 1475-2875

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