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Table 1 Potential role of targeted next generation sequencing in addressing malaria research of public health importance in Africa

From: Targeted Next Generation Sequencing for malaria research in Africa: current status and outlook

Scientific objective Application
Determination of the frequency and geographic distribution of genetic markers of antimalarial drug resistance Early detection of drug resistance emergence and surveillance of the geospatial changes of antimalarial drug resistance
Diversity, frequency and geographic distribution of genetic differences in potential vaccine antigens among malaria parasites, Support for antigen diversity studies in vaccine development and prediction of vaccine efficacy
Distribution and quantitative level of parasite DNA in human populations Estimation of prevalence of submicroscopic infection
Determination of efficacy of diagnostic tools Surveillance for geospatial distribution of parasites carrying hrp2/3 deletion
Determination of RDT level of sensitivity
Detection of full range of parasite genotypes in a patient isolate Increased sensitivity to determine whether parasites recurring after drug treatment are new infections or recrudescence/relapse of initial infections
Detection of very low levels of parasite DNA in patient samples Improved detection of parasite prevalence even at low parasitaemia
Improved quantitation of delayed parasite clearance